SNP Calling Improve this page

#http://www.htslib.org/workflow/#mapping_to_variant
set -xeu

FQ1=y1.fastq
FQ2=y2.fastq
REF=yeast.fasta
BNM=yeastD

RUNINDOCKER=1

SAMTOOLS=samtools
BWA=bwa
TABIX=tabix
BCFTOOLS=bcftools
PLOTVCFSTATS=plot-vcfstats

if [[ "$RUNINDOCKER" -eq "1" ]]; then
echo "RUNNING IN DOCKER"
DRUN="docker run --rm -v $PWD:/data --workdir /data -i"
#--user=biodocker

SAMTOOLS_IMAGE=/samtools
BWA_IMAGE=/bwa
TABIX_IMAGE=dev/htslib:1.2.1
BCFTOOLS_IMAGE=/bcftools


docker pull $SAMTOOLS_IMAGE
docker pull $BWA_IMAGE
docker pull $TABIX_IMAGE
docker pull $BCFTOOLS_IMAGE

SAMTOOLS="$DRUN $SAMTOOLS_IMAGE $SAMTOOLS"
BWA="$DRUN $BWA_IMAGE $BWA"
TABIX="$DRUN $TABIX_IMAGE $TABIX"
BCFTOOLS="$DRUN $BCFTOOLS_IMAGE $BCFTOOLS"
PLOTVCFSTATS="$DRUN $BCFTOOLS_IMAGE $PLOTVCFSTATS"
else
echo "RUNNING LOCAL"
fi

HEADLEN=100000

if [[ ! -f "$FQ1" ]]; then
curl ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR507/SRR507778/SRR507778_1.fastq.gz| gzip -d | head -$HEADLEN > $FQ1.tmp && mv $FQ1.tmp $FQ1
fi

if [[ ! -f "$FQ2" ]]; then
curl ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR507/SRR507778/SRR507778_2.fastq.gz| gzip -d | head -$HEADLEN > $FQ2.tmp && mv $FQ2.tmp $FQ2
fi

if [[ ! -f "$REF" ]]; then
curl ftp://ftp.ensembl.org/pub/current_fasta/saccharomyces_cerevisiae/dna/Saccharomyces_cerevisiae.R64-1-1.dna_sm.toplevel.fa.gz | gunzip -c > $REF.tmp && mv $REF.tmp $REF
fi

if [[ ! -f "$REF.fai" ]]; then
$SAMTOOLS faidx $REF
fi

if [[ ! -f "$REF.bwt" ]]; then
$BWA index $REF
fi

if [[ ! -f "$BNM.sam" ]]; then
$BWA mem -R '@RG\tID:foo\tSM:bar\tLB:library1' $REF $FQ1 $FQ2 > $BNM.sam.tmp && mv $BNM.sam.tmp $BNM.sam
fi

if [[ ! -f "$BNM.bam" ]]; then
#$SAMTOOLS sort -O bam -T /tmp -l 0 --input-fmt-option SAM -o $BNM.tmp.bam $BNM.sam && mv $BNM.tmp.bam $BNM.bam
$SAMTOOLS sort -O bam -T /tmp -l 0 -o $BNM.tmp.bam $BNM.sam && mv $BNM.tmp.bam $BNM.bam
fi

if [[ ! -f "$BNM.cram" ]]; then
$SAMTOOLS view -T $REF -C -o $BNM.tmp.cram $BNM.bam && mv $BNM.tmp.cram $BNM.cram
fi

if [[ ! -f "$BNM.P.cram" ]]; then
$BWA mem $REF $FQ1 $FQ2 | \
$SAMTOOLS sort -O bam -l 0 -T /tmp - | \
$SAMTOOLS view -T $REF -C -o $BNM.P.tmp.cram - && mv $BNM.P.tmp.cram $BNM.P.cram
fi

#if [[ ! -f "" ]]; then
#$SAMTOOLS view $BNM.cram
#fi

#if [[ ! -f "" ]]; then
#$SAMTOOLS mpileup -f $REF $BNM.cram
#fi

if [[ ! -f "$BNM.vcf.gz" ]]; then
$SAMTOOLS mpileup -ugf $REF $BNM.bam | $BCFTOOLS call -vmO z -o $BNM.vcf.gz.tmp && mv $BNM.vcf.gz.tmp $BNM.vcf.gz
fi

if [[ ! -f "$BNM.vcf.gz.tbi" ]]; then
$TABIX -p vcf $BNM.vcf.gz
fi

if [[ ! -f "$BNM.vcf.gz.stats" ]]; then
$BCFTOOLS stats -F $REF -s - $BNM.vcf.gz > $BNM.vcf.gz.stats.tmp && mv $BNM.vcf.gz.stats.tmp $BNM.vcf.gz.stats
fi

mkdir plots &>/dev/null || true

#if [[ ! -f "plots/tstv_by_sample.0.png" ]]; then
#$PLOTVCFSTATS -p plots/ $BNM.vcf.gz.stats
#fi

if [[ ! -f "$BNM.vcf.filtered.gz" ]]; then
$BCFTOOLS filter -O z -o $BNM.vcf.filtered.gz -s LOWQUAL -i'%QUAL>10' $BNM.vcf.gz
fi